Internal Structure of Apoferritin as Revealed by the "Negative Staining Technique."

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Internal Structure of Apoferritin as Revealed by the "Negative Staining Technique."

I t has been surmised that the apoferritin molecule has a hollow center which communicates with the outer surface through some sort of channels or slots (1-2). This inference is based upon the following findings: 1. As determined by means of electron microscopy, the ferric hydroxide (FeOOH) micelles of ferritin molecules are located within spheroids of apoferritin (1) ; 2. In reducing media, su...

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Sucrose-gradient-purified dictyosomes of plant Golgi apparatus appear, after glutaraldehyde stabilization, as stacks of highly fenestrate and tubate cisternae when negatively stained with phosphotungstic acid, shadowed with heavy metal, or OsO(4)-stained in thin section. The tubular proliferations (diameter 200 to 400 A) extend for several microns from the central region and are united at inter...

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ژورنال

عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology

سال: 1959

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.6.3.531